Water Pepper Rivaled a Chemical Spray on Rice Grasshoppers
Lemnsah Andrew Banboye, Konkuk University, Department of Bioresources and Food Science (MS student; supervisors Prof. Se Chul Chun and Prof. Doo Hwan Kim) · Cameroon
Two one-season rice trials tested boiled plant extracts as insect sprays. On Konkuk's natural farming paddy in Paju, water pepper, tobacco leaf and neem sprays cut pest counts to a fraction of the unsprayed plots while dragonflies stayed; in the lab, water pepper and tobacco killed every grasshopper within two days. On a conventional farm in Yeoju, water pepper extract held grasshoppers and stink bugs about as low as the chemical cypermethrin, but not stem borers.
What they found
Can boiled plant extracts (chili pepper, lemon peel, water pepper, tobacco leaf, neem oil) control rice insect pests, and how do they compare with a chemical insecticide (cypermethrin)?
2016 season (dates not stated). Lab screen: 6 cages of 10 green grasshoppers; each extract sprayed onto one cage for 2 seconds, one untreated cage; survival read at 1 h, 2 h, 1 day and 2 days (one cage per treatment, no replication). Experiment I, Paju (Konkuk natural farming paddy, same field as the previous speaker's): 0.2 ha swamp rice plot, soil amended with 0.4 t IMO, Chucheong variety transplanted at 30 x 15 cm, 8-15 seedlings per hill; water control, hand weeding; nutrients from natural farming solutions (FAA, BRV, LAB, WSCP, seawater, WSC and minerals; one item hidden). 24 plots of 5 x 5 m in 2 blocks of 12; 6 treatments x 4 replicates, RCBD; sprays at 15, 30, 45, 60, 75 and 90 days after transplanting; 12 hills per plot measured. Experiment II, Yeoju (conventional farm): Hopum variety machine-transplanted at the same spacing; 4.5 kg NPK at 14 days and 4.5 kg N at 80 days (area not stated); herbicides for weeds; 5 treatments x 4 replicates, RCBD; sprays at 21, 42, 63 and 84 days. Statistical test not named; slides show significance letters. Grain yield not reported.
- Chili pepper extract (Capsicum annuum) — Plant material boiled 8 hours, diluted 1:15, foliar spray. Active molecule per slide: capsaicin. Both experiments.
- Lemon peel extract (Citrus limon) — Boiled 8 hours, diluted 1:15, foliar spray. Limonene. Lab and Paju only.
- Water pepper extract (Persicaria longiseta, Korean 'yeokkwi') — Boiled 8 hours, diluted 1:15, foliar spray. Both experiments.
- Tobacco leaf extract (Nicotiana tabacum) — Boiled 8 hours, diluted 1:15, foliar spray. Nicotine. Lab and Paju only.
- Neem oil (Azadirachta indica) — 1:5000 in Yeoju; Paju dilution not stated separately. Azadirachtin.
- Pyretan (cypermethrin), chemical — 1:1000, Yeoju only.
- Control — No insecticide; whether plots were sprayed with water is not stated.
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Lab: water pepper and tobacco killed all grasshoppers within 2 days
Table 1, % surviving after 1 h / 2 h / 1 day / 2 days: tobacco 60/20/10/0; water pepper 80/70/50/0; chili 80/80/60/20; neem 100/100/80/20; lemon peel 100/100/100/60; control 100/100/100 (2-day cell hidden; spoken: all alive).
▶ 6:04 Said and shown on the slides
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Paju: rice water weevils 1.7-12.5 per plot with extracts vs 17.0 untreated
Table 2: neem 1.7 (e), tobacco 4.2 (d), water pepper 5.5 (d), chili 7.7 (c), lemon 12.5 (b), control 17.0 (a).
▶ 7:16 Said and shown on the slides
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Paju: green grasshoppers 2.2 per plot with water pepper vs 13.7 untreated
Table 2: water pepper 2.2 (d), neem 3.5 (cd), tobacco 3.7 (cd), chili 5.5 (bc), lemon 7.5 (b), control 13.7 (a).
▶ 7:16 Said and shown on the slides
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Paju: stem borers and stink bugs also lower with every extract
Table 2: stem borers 1.2-2.5 vs 3.5 control; rice stink bugs 0.7 (tobacco) to 2.2 vs 3.2 control. Water pepper's stink-bug cell reads 2.2 with letter b while lemon's 2.2 carries ab.
▶ 7:23 From the slides
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Dragonflies were not reduced
Table 2: 1.0-2.2 per plot with extracts vs 2.7 control, all letter a. The speaker also saw many spiders; spiders were not counted on the slide.
▶ 7:46 Said and shown on the slides
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Water pepper gave shorter plants but the most panicles
Table 3: height 83.5 cm (b) vs 89.7-93.0 (a) for all others; tillers 20.0 vs 15.0 control (all extracts 17.5-20.0 share letter a); fertile panicles 19.7 (a) vs 14.2 (c) control, 16.2-18.2 for other extracts. Every extract raised tillers and panicles over the control. Shorter plants lodge less, the speaker says.
▶ 8:13 Said and shown on the slides
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Yeoju: water pepper matched cypermethrin on grasshoppers and stink bugs
Table 4, insects per plot: green grasshopper cypermethrin 2.2 (c), water pepper 2.7 (c), neem 3.5 (bc), chili 5.5 (b), control 8.0 (a); rice stink bug 2.2 (c), 2.5 (bc), 3.5 (bc), 4.2 (b), control 6.2 (a).
▶ 8:58 Said and shown on the slides
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Yeoju: water pepper did not control stem borer; cypermethrin did
Table 4, stem borer: cypermethrin 0.7 (b), water pepper 2.2 (a), neem 2.0 (ab), chili 1.7 (ab), control 2.7 (a). Brown grasshopper: cypermethrin 0.5 (c), water pepper 1.2 (bc), control 2.7 (a). The talk's 'similar performance' holds for two of four pests.
▶ 9:07 From the slides
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No beneficial insects in the chemical (conventional) plots, per the speaker
Slide footnote: cypermethrin is non-selective, 'no beneficial insects here as in natural farming plot Table 2'. Table 4 has no beneficial-insect column, so this was not counted.
▶ 9:15 Said and shown on the slides
- One season; each experiment at one site.
- Lab screen: one cage of 10 grasshoppers per treatment, no replication.
- No grain yield was reported, though the conclusion says the extracts 'can improve rice productivity'.
- Paju and Yeoju differ in farm system, variety, fertilizer and spray schedule, so the two experiments cannot be compared directly; lemon and tobacco were not tested in Yeoju.
- The beneficial-insect claim for chemical plots is not backed by counts on the slides.
- Statistical test not named; one Table 2 cell has inconsistent letters.
- Spoken 'five times' for the Paju sprays; the slide lists six dates.
- Tobacco extract is nicotine, itself a broad nerve poison (the slide notes it interrupts cholinesterase); safety and residues were not tested.
- Boiled water pepper, tobacco leaf and neem sprays helped keep rice pests far below unsprayed plots in this trial, while dragonflies stayed.
- Water pepper extract at 1:15 held grasshoppers and stink bugs about as low as cypermethrin; for stem borer it did not.
- Recipe used: boil the plant material 8 hours, dilute 1:15, spray leaves about every 15-21 days.
- Water pepper-treated rice was shorter yet carried the most panicles; shorter rice lodges less.
Next to Master Cho's teaching
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Agrees
Boiled hot-pepper water as a spray Cho's hot-pepper water boils 10-12 peppers in 1 L down to half, then mixes it into 20 L of water, to repel aphids; the trial's boiled chili extract is the same idea at a different ratio (1:15) and against different pests.
Soap-water-and-Hot-pepper-water, p. 2–7
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Differs
Kill pests vs grow plants they don't attack Cho says it matters more to grow plants that pests don't attack in the first place; pests follow excess nitrogen and nutrient imbalance. The talk tests sprays to kill insects and does not look at plant condition.
Cho recipe book (scan), p. 30
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Agrees
Repel, don't spray indiscriminately, on rice Cho warns that harsh sprays harm soil life and, for rice leaf roller and stem borer, prefers repellents over blanket spraying; the trial's point that plant extracts left dragonflies, while the chemical left no beneficials, fits this.
2017/Advanced Cho/advanced fruit/IMG_3201 @ 1:39:50 (translated)
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Agrees
Tobacco against insects Cho's book (read in Pure KNF Office Hours) lists tobacco as the usual material for the herbal smoker against greenhouse pests; the trial used it as a boiled leaf spray instead.
Office Hours/Recordings - Video/oh116-240310 @ 0:55:29
“When the world will change and adopt natural farming, surely insects and other pests will also change the strategy to survive.”
▶ 1:34
“One thing to remark is that there was the presence of beneficial insects. We had a lot of dragonflies in the field, and spiders.”
▶ 7:46
“Water pepper in particular had a similar performance to cypermethrin, a chemical pesticide.”
▶ 8:58
In this talk
- 0:00Intro
- 0:45Rice insect pests
- 1:18Why natural pesticides
- 1:56Two experiments: Paju and Yeoju
- 2:23The five plant extracts
- 3:48Lab screen on grasshoppers
- 4:13Paju field trial design
- 4:54Yeoju conventional trial
- 6:04Lab results
- 7:08Field pest counts
- 8:04Plant height, tillers, panicles
- 8:45Water pepper vs cypermethrin
- 9:31Conclusion
Transcript
Transcribed and checked by hand against the recording. Click any time to play from there.
0:00 My name is Andrew and I am from Cameroon. This afternoon I will present to you my research on the comparison of chemical and bio insecticide in rice. This work has been supervised by Professor Se Chul Chun and Professor Doo Hwan Kim. My colleague has just presented the same rice where I am carrying out my experiment. So to save much time, I won't go into the details of the rice production, but I will present to you the technical aspects and the results of my experiment. So the rice crop is highly attacked by rice insects that generally cause more than 35%
0:51 yield losses, and these insects include the rice water weevil and termites, which are predominant root insects, and the stem borers that attack the stems. Meanwhile we have the leafhoppers, planthoppers and larvae and adults of beetles and grasshoppers that generally attack the leaves, and the stink bug is the common enemy to the rice grain. It's an example of a stink bug. So to test these pesticides, since morning we have been talking on the techniques of improving sustainable food. That is food that is environmentally friendly and healthy. All along we have been developing strategies. But
1:34 when the world will change and adopt natural farming, surely insects and other pests will also change the strategy to survive and how to move. They will also change the way they enter crops, and our natural farming products will be in danger. So it is high time we develop natural pesticides to ensure that our products will survive in the future. So to do this, I carried out two experiments. One was in Paju on natural rice farming and one was in Yeoju. So at the end, the results were compared and then I will present what I found.
2:12 So in experiment one, as I said my colleague was already presented the experiment on rice, so I will present only the salient points of the experiment. So in experiment one, we used bio-extracts which were obtained by boiling plant samples for 8 hours, diluting them to 1 to 15 ratio and applying as foliar sprays. And the following treatments we use: the plant extracts we use are red pepper and then lemon peels, from which we obtain the insecticide.
2:58 Then Persicaria longiseta, which is commonly called water pepper, and in Korea they call it yeokkwi. We equally use tobacco leaves. That is a tobacco. I just pay this chemical, this molecules, because I know maybe it will cause problems. These are the active ingredients in the chemicals that are responsible for the insecticidal effects of the products we use. So, I just present to you the plant species, and if there is someone who wants to know detail why the plant is an insecticide, maybe he can then follow up by reading more of it. And then the last one was neem oil.
3:47 So, when we obtain these extracts, we first tested the extracts in the laboratory to make sure that the extracts have insecticidal effects, that is that they could kill insects. And this was done by introducing 10 insects in a cage and then applying the extracts from the plants directly on the insects and observing them after some time and data recording. After carrying out the laboratory screening to make sure that the pesticides, the plant plant extracts are really insecticides, we now carried out a field trial where we tested these
4:24 plant extracts on the field and to do this we used 24 experimental plots of five by five meters each in two blocks of 12, and we applied six treatments replicated four times following a randomized complete block design, and treatments were applied at 15 ... 45, 60, 90 days, that's five times, and in the field we selected 12 hills per plot for growth data collection. Meanwhile, in experiment two, on conventional farming, that is where we use chemicals, we transplanted seedlings of Hopum rice variety at this density, and we supplied plant nutrients
5:08 using chemical fertilizers. We applied 4.5 kilograms of NPK fertilizers at 14 days after transplanting and 4.5 kilograms of nitrogenous fertilizers at 80 days after transplanting. We equally used herbicides for weed control. So these are the treatments we use at the conventional farm. We use a popular chemical insecticide in Korea known as Pyretan, which we diluted at 1 to 1000, and the active ingredient is cypermethrin. And then the second treatment was water pepper, or yeokkwi. The third treatment was neem oil and the fourth treatment was chili pepper. Those are the
5:56 concentrations we used with the chemicals. And we applied to the plants on these days, four times. Then we observed that in the laboratory evaluation screening test, laboratory evaluation of bio-pesticides, water pepper and tobacco leaves performed best, because after treating them with these substances for two days, all the insects in the cage where water pepper and tobacco were used, all of the insects died. But 20 percent of the insects were still remaining in the cage treated with chili and neem oil, while 60 percent of the insects were remaining in
6:46 the cage treated with lemon peel extracts. And in the control cage, where no insecticide was applied, all the insects were living, no insect died, showing that really the plant extracts we are using have insecticidal effects, that they can successfully kill insects. Then we proceeded to the field, and this was the result we obtained. In the field, after treatment, we observed that water pepper, tobacco leaves and neem oil were efficient in controlling these rice insect pests, that is rice water weevil, green grasshopper, rice stem borer, rice
7:30 stink bugs, than chili pepper and lemon. So from there we can see that these three extracts, water pepper, tobacco and neem, have better insecticidal properties. And in this field, one thing to remark is that there was the presence of beneficial insects. That is, we had a lot of dragonflies in the field, and spiders. We go ahead, you see the other piece. Then, when we compare the effects of these plant extracts on the growth of rice, we noticed that the plot treated with water pepper again produced shorter plants.
8:19 My friend just showed some plants which were lodged and some which were standing. So we see that when plants are very tall, they are not very good in rice because they can easily lodge. But water pepper produces shorter plants. And paradoxically, these shorter plants produce the highest number of tillers, as you can see, and the highest number of panicles than all other pesticides tested. So now, in experiment two, where we used the chemical insecticide cypermethrin and the other bio-pesticides,
8:58 we observed that water pepper in particular had a similar performance to cypermethrin, a chemical pesticide. That means water pepper can be substituted to cypermethrin if we can use it and have almost the same results. And when we look at the percentage of insects in the field, we see that in the plot where we use conventional, there are no beneficial insects which proves that the chemical pesticides are dangerous to the environment, to insects and to human beings as a whole. And that is why, in conclusion, we can proudly say now that
9:38 natural pesticides can successfully replace chemical pesticides and that neem oil, water pepper and tobacco leaves extract have promising insecticidal effects and can improve rice productivity in Asia and in other countries. Thank you.